Quantitative profiles of *C. elegans* locomotive behaviors

**Locomotion parameters**

Parameters |
Unit |
Definition |

Velocity_FWD | mm/sec | Speed of point traveling along the worm’s track in forward movement |

Velocity_BWD | mm/sec | Speed of point traveling along the worm’s track in backward movement |

Centroid Velocity_FWD | mm/sec | Change in centroid position over time in forward movement |

Centroid Velocity_BWD | mm/sec | Change in centroid position over time in backward movement |

Frequency_FWD | Hz | Oscillations per second for each articulation point. This is a set of 11 parameters. This parameter measures the frequency of body bending in forward movement |

Frequency_BWD | Hz | Oscillations per second for each articulation point. This is a set of 11 parameters. This parameter measures the frequency of body bending in backward movement |

Flex_FWD | rad | Maximum angle difference between ventral and dorsal bend for each articulation point. This parameter measures the extent of body bending in forward movement. This is a set of 11 parameters |

Flex_BWD | rad | Maximum angle difference between ventral and dorsal bend for each articulation point. This parameter measures the extent of body bending in backward movement. This is a set of 11 parameters |

Time Delay_FWD | sec | The time required for one point to reach the same angle as its adjacent anterior neighbor. This parameter measures the speed of wave propagation along the body in forward movement. This is a set of 10 parameters |

Time Delay_BWD | sec | The time required for one point to reach the same angle as its adjacent anterior neighbor. This parameter measures the speed of wave propagation along the body in backward movement. This is a set of 10 parameters |

Amplitude_FWD | mm | Width of the best-fit bounding box aligned along the worm in forward movement |

Amplitude_BWD | mm | Width of the best-fit bounding box aligned along the worm in backward movement |

Wavelength_FWD | mm | Wavelength of the sine wave fitted along the worm posture in forward movement |

Wavelength_BWD | mm | Wavelength of the sine wave fitted along the worm posture in backward movement |

Length | mm | The length of the midline of the worm |

Duration of FWD movement | sec | Total forward moving time |

Duration of BWD movement | sec | Total backward moving time |

Duration of stopped time | sec | Total stopped time |

Reversals | per minute | Average number of reversals per minute |